Containment of Microbial Aerosols in a Microbiological Safety Cabinet

Containment of microbial aerosols in a microbiological safety cabinet.

A microbiological safety cabinet was evaluated to determine conditions under which microorganisms might escape. Tests were conducted under three cabinet-closure conditions, various airflow velocities, and different laboratory operations, with 10(5), 1.1 x 10(5), and 10(6) microorganisms per cubic foot of cabinet space released per min for 5 min. The data revealed that (i) escape of a human infe...

Effects of airflow rates and operator activity on containment of bacterial aerosols in a class II safety cabinet.

Biological safety cabinets are frequently relied upon to provide sterile work environments in which hazardous microorganisms can be safely handled. Verification of correct airstream velocities does not, by itself, ensure that adequate protection will be achieved under all users. Instead, the concentration of microorganisms in a cabinet operator's breathing zone must be measured during typical c...

Assessing the containment efficiency of a microbiological safety cabinet during the simultaneous generation of a nanoaerosol and a tracer gas.

The intention of this article is to compare the containment performance of a Type II microbiological safety cabinet (MSC) confronted with the simultaneous generation of a saline nanoparticle aerosol and a tracer gas (SF(6)). The back dissemination coefficient, defined as the ratio of the pollutant concentration measured outside the enclosure to the pollutant flow rate emitted inside the enclosu...

HEPA EXHAUST FILTER IV HEPA SUPPLY FILTER I / All

A microbiological survey of the effectiveness of class II (laminar flow) 'safety' cabinets found in the UK in the last six years is recorded. Only two of the nine units tested approached the containment of aerosols achieved by a good class I (exhaust protective) cabinet. The others were potentially hazardous if used with pathogenic material. The National Sanitation Foundation and the British St...

Policy on Choice of Fumigant for Fumigation of Microbiological Safety Cabinets and Containment Level 3 Suites